SrasV1, Main, Exploration, bibRecord, 005B14

Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells

Identifieur interne : 005B14 ( Main/Exploration ); précédent : 005B13; suivant : 005B15

Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells

Auteurs : ZHI WANG [République populaire de Chine] ; Lili Ren [République populaire de Chine] ; XINGANG ZHAO [République populaire de Chine] ; TAO HUNG [République populaire de Chine] ; ANMING MENG [République populaire de Chine] ; JIANWEI WANG [République populaire de Chine] ; YE-GUANG CHEN [République populaire de Chine]

Source :

Journal of virology [ 0022-538X ] ; 2004.

RBID : Pascal:04-0393340

Descripteurs français

Pascal (Inist)
- Virus, Mammalia, Réplication, Silence expression génique, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Gene silencing, Mammalia, Microbiology, Replication, Severe acute respiratory syndrome, Virology, Virus.

Abstract

Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.

Affiliations:

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to stream PascalFrancis, to step Corpus: 000845
to stream PascalFrancis, to step Curation: 000145
to stream PascalFrancis, to step Checkpoint: 000824
to stream Main, to step Merge: 005F75
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Le document en format XML

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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.</div>
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Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells

Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri